NY96015 - Part 1 - Detection and characterisation of orchid fleck virus

The main objective of this project was to develop a nucleic acid based diagnostic system for orchid flect virus (OFV). OFV is one of three commonly found viruses infecting orchids. Diagnosis of virus diseases are commonly conducted by immunological tests but this method is not available for use with OFV because OFV has a very unstable virion. Current laboratory tests used for diagnosis of OFV involve the examination of leaf sap by electron microscopy. This is time consuming, costly and my unreliable because OFV is often present in very low concentrations. A molecular based detection system will offer a rapid, accurate and cost effective test for the diagnosis and identification of OFV in infected plant samples.